Inclusion Criteria

- INCLUSION CRITERIA:

Patients must have gp100 reactive cells obtained and evaluated while participating in the
Surgery Branch protocol, 'Cell Harvest and Preparation for Surgery Branch Adoptive Cell
Therapy Protocols' or on another IRB approved Surgery Branch adoptive cell therapy study,
i.e. 99-C-0158 or 03-C-0162.

Patients must be greater than or equal to 16 years of age and must have measurable
metastatic melanoma that is refractory to standard therapy, or has relapsed after standard
therapy, including high dose IL-2 therapy.

Patients must be HLA-A*0201 positive.

Patients of both genders must be willing to practice birth control during treatment and
for four months after receiving the preparative regimen.

Clinical performance status of ECOG 0, 1.

Absolute neutrophil count greater than 1000/mm3 without support of filgrastim.

Platelet count greater than 100,000/mm3.

Hemoglobin greater than 8.0 g/dl (can be corrected with transfusion).

Serum ALT/AST less than three times the upper limit of normal.

Serum creatinine less than or equal to 2.0 mg/dl.

Total bilirubin less than or equal to 2.0 mg/dl, except in patients with Gilbert's
Syndrome who must have a total bilirubin less than 3.0 mg/dl.

Must be willing to sign a durable power of attorney.

Patients must be able to understand and sign the Informed Consent document.

Patients with resected brain metastases will be eligible.

Patients who are to receive high dose IL-2 and who are 50 years old or greater must have a
normal stress cardiac test (stress thallium, stress MUGA, dobutamine echocardiogram, or
other stress test) with an LVEF greater than 45 percent.

Patients who are to receive high dose IL-2 who have history of EKG abnormalities, symptoms
of cardiac ischemia or arrythmias must have a normal stress cardiac test (stress thallium,
stress MUGA, dobutamine echocardiogram, or other stress test) with an LVEF greater than 45
percent.

Patients who are to receive high dose IL-2 who have a prolonged history of cigarette
smoking or symptoms of respiratory dysfunction must have a normal pulmonary function test
as evidenced by a FEV(1) greater than 60 percent predicted.

CELL INFUSION EXCLUSION CRITERIA:

Less than four weeks has elapsed since any prior systemic therapy at the time the patient
receives the preparative regimen, or less than six weeks since prior nitrosurea therapy.
All patients' toxicities must have recovered to a grade 1 or less or as specified in the
above eligibility criteria. Patients may have undergone minor surgical procedures with
the past 3 weeks, as long as all toxicities have recovered to grade 1 or less or as
specified in the eligibility criteria in section 2.1.1.

Women of child-bearing potential who are pregnant or breastfeeding because of the
potentially dangerous effects of the preparative chemotherapy on the fetus or infant.

Life expectancy of less than three months.

Systemic steroid therapy required.

Any active systemic infections, coagulation disorders or other major medical illnesses of
the cardiovascular, respiratory or immune system, as evidenced by a positive stress
thallium or comparable test, myocardial infarction, cardiac arrhythmias, obstructive or
restrictive pulmonary disease.

Any form of primary or secondary immunodeficiency. Must have recovered immune competence
after chemotherapy or radiation therapy as evidenced by normal lymphocyte counts greater
than 500 (grade 3 toxicity), normal ANC greater than 1000/mm3 and absence of opportunistic
infections. (The experimental treatment being evaluated in this protocol depends on an
intact immune system. Patients who have decreased immune competence may be less
responsive to the experimental treatment and more susceptible to its toxicities.)

Seropositive for HIV antibody. (The experimental treatment being evaluated in this
protocol depends on an intact immune system. Patients who are HIV seropositive can have
decreased immune competence and thus be less responsive to the experimental treatment and
more susceptible to its toxicities.)

Patients with hepatitis B or hepatitis C will be excluded.

Seronegative for Epstein-Barr virus (EBV).

Allergy to eggs or any known hypersensitivity to any known agents on this trial.

Patients who are not willing to complete a DPA will be excluded.