The overall objective is to determine whether the predictive value of plasma concentrations
of selected nutrient biomarker(s) of food intake determined using a single plasma sample
either alone or in combination are stronger, objective predictors of subsequent death from
CHD or MI compared to selected food intake data derived from subjective, self-reported food
frequency questionnaires. The nutrient biomarkers (phospholipid [PL] eicosapentaenoic acid
[EPA], PL docosahexaenoic acid [DHA], PL trans fatty acids, phylloquinone,
dihydrophylloquinone) and foods (fish, dark fish and tuna, vegetables, fruits, and whole
grains, and unsaturated fat rich foods) targeted have previously been either directly or
indirectly associated with CVD risk. We propose to test our overall objective by conducting
a nested case-control study using plasma samples and food frequency data from the
observational cohort of the Women's Health Initiative (WHI). Our cases (n=1200) will be
selected from the subset of women who did not report dietary supplement use and who died of
CHD or MI (collectively referred to as WHI CHD cases). The control subjects (n=1200) will
be selected from the subset that were free of CHD or MI events and matched with cases for
standard National Cholesterol Education Program (NCEP) risk factors (WHI controls).
Nutrient biomarker data will be newly generated using stored specimens whereas the selected
food intake data have previously been collected by the WHI investigators.
Observational Model: Case Control, Time Perspective: Cross-Sectional
plasma markers of dietary intake
phospholipid fatty acid profiles
Alice H Lichtenstein, D.Sc.
United States: Institutional Review Board
|Tufts University||Boston, Massachusetts 02111|