Observational - Gene Discovery in Osteosarcoma
Study Subtype: Ancillary/Correlative Observational Study Model: Cohort Time Perspective:
Retrospective Biospecimen Retention: Samples With DNA Biospecimen Description: Tissue Study
Population Description: Samples from the COG Sampling Method: Non-Probability Sample
I. To use PET sequencing to analyze DNA from ten human primary osteosarcoma samples, along
with matched normal tissue where available.
Tissue samples are analyzed for translocations and deletions and analyzed using polymerase
chain reaction (PCR) or fluorescence in situ hybridization (FISH).
Intervention Model: Single Group Assignment, Masking: Open Label
Mapping and filtering of paired tags
Mapping of paired tags to reference genomes to identify aberrant pairs, caused by translocations, insertions, deletions and inversions.
Up to 2 years
Children's Oncology Group
United States: Institutional Review Board
|Children's Oncology Group||Arcadia, California 91006-3776|