Detection of Cancer-Specific Active Proteases in Blood Via Fluorescence
The goal of the project is to develop a rapid in vitro screening assay for detection of
cancer biomarkers in blood. The aim is testing and optimization of nanoparticle sensors,
based on cyanine dyes that are chemically linked to Fe/Fe3O4 nanoparticles via
protease-selective consensus (cleavage) sequences. The focus is on the quantitative
determination of active cancer-specific proteases in blood via simple fluorescence
measurements. The matrix metalloproteinases, urokinase-type plasminogen activator, and
cathepsins, are up-regulated in the vast majority of progressing cancers and can, therefore,
serve as markers for cell survival/tumor progression, angiogenesis, and tissue
remodeling/invasion
Observational
Time Perspective: Prospective
Stephen Williamson, MD
Principal Investigator
University of Kansas Medical Center Cancer Center
United States: Institutional Review Board
13421
NCT01707758
October 2012
February 2013
Name | Location |
---|