Tissue Microarray Studies of the 12q13-q14 Amplicon in Alveolar Rhabdomyosarcoma
- To investigate (in two stages) which proteins are overexpressed in association with
12q13-q14 amplification by applying fluorescence in situ hybridization (FISH) and
immunohistochemical (IHC) assays to a panel of alveolar rhabdomyosarcomas (ARMS) cases
organized into tissue microarrays.
OUTLINE: Formalin-fixed paraffin-embedded tissue samples are analyzed for 12q13-q14
frequency and protein overexpression by FISH and IHC.
Relationship of amplification and fusion status using Fisher's exact test and Freeman-Halton test
Frederick Barr, MD
NCI - Laboratory of Pathology
United States: Federal Government