Identification and Characterization of Putative Leiomyoma Stem Cells.
The organ of interest in our study is the myometrium, a muscle layer of the uterus located
between the perimetry and the endometrium.
The investigators try to identify, locate and characterize the myometrial stem cell
population in the uterine fibroids by isolation of "Side Population" by flow cytometry. Once
the investigators achieve the purposes to set out, the investigators would try to maintain
"in vitro" stem cell population isolated from myometrial fibroids, for further endocrine
characterization. Finally, the investigators would try to induce the formation of fibroids
in murine models as a first step to demonstrate that the origin of these formations is due
to the abnormal proliferation of myometrial adult stem cells. The results of this study will
allow the identification of the cell population in the fibroids. The investigators also can
being able to compare with the myometrium in order to establish a relationship that helps us
to understand more about the pathophysiology of this disease, using these stem cells as
targets therapy in the treatment of this myometrium condition.
Observational Model: Case-Only, Time Perspective: Prospective
Formation and growth of human leiomyomas
Assessment of pluripotent potential from isolated cells responsible for the formation and growth of human leiomyomas.
Spain: Ministry of Health