Inclusion Criteria:

- Patients > or = 18 years

- Patients with advanced melanoma stage IIIC (unresectable) or stage IV

- Patient not treated or not responding to chemotherapy with chemotherapy session last>
1 month

- Patients who signed informed consent

- Patients presenting no socio-economic, psychological, familial or geographical allow
proper understanding of the information leaflet of the protocol or the regular
monitoring in the department of dermatology

- Patients with a life expectancy greater than 3 months

- Patients with melanoma measurable by RECIST version 1.1

- Patients with venous good for venipuncture

Exclusion Criteria:

- Patients with contraindication for treatment with chemotherapy or V600E BRAF
inhibitor or ipilimumab or have conditions concomitant heavy may interfere with the
treatment of metastatic melanoma

- Pregnant women or nursing

- People vulnerable detainees, adults under guardianship or curatorship, minors.