Genomics of Small Cell Lung Carcinoma
- To identify the major genetic alterations in human small cell lung cancer (SCLC) using
next-generation sequencing and focusing on mutations in coding sequence.
- To follow up with in-depth assessment of candidate oncogenes and tumor suppressor genes
using gene expression analysis, cell culture systems, and murine models of SCLC.
OUTLINE: DNA isolated from archived tumor tissue and blood samples are analyzed for genetic
mutations and gene expression profiling using Illumina exome sequencing. Results are
compared with transcriptome of tumors (or cell lines) with and without the specific
mutation. Cell culture studies overexpressing or inhibiting the genes of interest are also
performed in a mouse model to identified potential drivers of small cell lung cancer.
Identification of genetic alterations in human SCLC (driver or passenger mutations)
David MacPherson, PhD
Carnegie Institution for Science