Diagnosing Invasive Aspergillosis by PCR Based Investigation of Bronchoalveolar Lavage Samples During Antifungal Therapy
Invasive pulmonary aspergillosis (IPA) remains a major cause for morbidity and mortality in
patients (pts) with hematologic malignancies. As culture-based methods only yield results in
a minority of patients, using non-culture-based methods for detection of aspergillosis in
clinical specimens becomes increasingly important. Analyzing bronchoalveolar lavage (BAL)
samples with polymerase chain reaction (PCR) is promising, however, the influence of current
antifungal drugs on the performance of this diagnostic tool remains controversial.
The aim of the trial is to elucidate on the performance of BAL PCR under antifungal
Patients with high risk of invasive aspergillosis and lung infiltrates are sampled via BAL,
the sample is analyzed for fungal DNA by Apsergillus specific PCR. Clinical data including
treatment data is assessed and evaluated.
Observational Model: Case Control, Time Perspective: Retrospective
Dieter Buchheidt, MD
Mannheim University Hospital
Germany: Ethics Commission
Aspergillus PCR BAL Study