Xenotransplantation of Primary Leukemia Samples Into Zebrafish
- Demonstrate the capability of primary human leukemia samples to survive and proliferate
in the zebrafish embryo.
- Confirm the anti-proliferative or toxic effects of known chemotherapeutics on the
transplanted cells in vivo.
- Evaluate the effect of novel anticancer drugs and/or their combinations on individual
OUTLINE: This is a multicenter study.
Cryopreserved specimens are injected into the yolk sac of zebrafish embryos under
anesthesia. After 1 hour of recovery at 28° C, embryos are maintained at 38° C and screened
for fluorescence at the injection site. Within 48 hours post-injection, some embryos are
treated with various chemotherapeutic drugs or dimethyl sulfoxide and incubated in protease
solution. Proliferation of leukemia cells are monitored by live-cell microscopy. Cells with
or without drug treatment are then extracted at 24 and 72 hours post-injection. Leukemia
cells are counted and tested with imatinib mesylate, all-trans retinoic acid (tretinoin),
cytosine arabinose, and known bioactive chemical compounds from promising drug families,
such as tyrosine kinase inhibitors, antiapoptotic agents, channel modulators, and
Primary human leukemia samples capable to survive and proliferate in the zebrafish embryo
Jason N. Berman, MD
IWK Health Centre
United States: Federal Government