Trial Information

Up to 10 archival breast tumor blocks will be obtained from the Department of Pathology, NUH
- half of these blocks will be from patients who were diagnosed before year 2000, and the
other half of the blocks will be from patients diagnosed between year 2000-2005 (control
specimens). Basic tumor and patient information will be collected without patient
identifiers - grade, stage, ER/PR/HER2 status, treatment received, outcome data. 8-10
ten-micron sections will be cut from each tumor block. RNA will be extracted from the tumor
sections using the Roche High Pure FFPE RNA Micro Kit. RNA will be profiled using the
Illumina Veracode assay and the Affymetrix Quantigene assay. RNA extraction and gene
expression profiling will be performed by collabroators at the Genome Institute of
Singapore.