Assessment of Stem Cell Heterogeneity in AML in Co-Culture Systems Using X Chromosome Inactivation Patterns
- Determine the frequency of acute myeloid leukemia (AML) that originates in CD33+
precursors or in which uncontrolled growth is restricted to CD33+ precursors.
OUTLINE: Cryopreserved acute myeloid leukemia cell samples are separated from endothelial
cell by fluorescent-activated cell sorting (FACS) and analyzed for X-chromosome inactivation
patterns by cytogenetic/molecular analysis and/or Humara assay.
Frequency of AML that originate in CD33+ or in which uncontrolled growth is restricted to CD33+ precursors
Roland Walter, MD, PhD
Fred Hutchinson Cancer Research Center
United States: Federal Government