Stat3 Signaling Pathway Aberrancies in Pediatric AML
- Determine the frequency of ligand-independent phosphorylation of Stat3 in a small
cohort of samples from pediatric patients with acute myeloid leukemia including, but
not limited to, samples known to express mutated c-kit.
- Measure the level of Stat3 phosphorylation in these samples after stimulation of three
key cytokine receptors expressed on hematopoietic cells IL-6R, G-CSFR, and c-kit.
OUTLINE: Cryopreserved samples are analyzed for levels of various components of the Stat3
pathway via western blotting.
Correlations between levels of pY-Stat3 and levels of upstream cytokine receptors
Michele S. Redell, MD, PhD
Texas Children's Cancer Center
United States: Federal Government