Implications of s-SHIP Expression and SHIP Alterations in AML
- Determine the extent and prognostic implications of variable s-SHIP expression and SHIP
gene mutations in pediatric patients with acute myeloid leukemia.
- Determine the effects of aberrant s-SHIP expression on the PI3K/Akt pathway in these
patients via electrochemiluminescence and phosphospecific flow cytometry assays.
OUTLINE: RNA samples are analyzed for variable s-SHIP expression and are screened for SHIP
mutations. Cryopreserved cells (with known high- or low-s-SHIP expression) are analyzed for
the levels of various components of the PI3K/Akt pathway via electrochemiluminescence and
phosphospecific flow cytometry.
Phoenix Ho, MD
Fred Hutchinson Cancer Research Center
United States: Federal Government