Stat3 Activation as a Potential Prognostic Marker and Therapeutic Target in Pediatric AML
- To characterize Stat3 signaling pathway activity in primary tissue samples from
pediatric patients with acute myeloid leukemia to determine the percentage of samples
with increased activity and to better understand the mechanisms leading to increased
- To evaluate the presence of constitutive Stat3 activation and the sensitivity of Stat3
activation to low and high doses of cytokines.
- To evaluate the expression levels of Stat3 protein as well as upstream and downstream
regulators of Stat3 activation.
- To classify tissue samples according to a Stat3-activation pattern, and to correlate
this result with event-free survival and overall survival in order to determine whether
increased Stat3 phosphorylation at diagnosis predicts poor outcome.
OUTLINE: Cryopreserved cell samples are collected for laboratory analysis, including
immunoblotting, fluorescence activated cell sorting (FACS), and protein analysis.
Correlation of the Stat3 response category (constitutive, low-dose responsive, high-dose responsive, or unresponsive) with clinical data, including event-free and overall survival
Michele S. Redell, MD, PhD
Texas Children's Cancer Center
United States: Federal Government