Molecular Epidemiology of Esophageal Cancer: Pilot Project
- Examine the role of several genetically-determined factors in combination with
cigarette smoking and diet in the etiology and prevention of esophageal cancer.
- Identify polymorphisms in metabolizing enzymes (e.g., phase I or II metabolism [GSTM1,
GSTT1,CYP1A1, CYP3A5, mEH, NQO1, GSTP1], DNA repair [XRCC1, ERCC2], free-radical
formation [MPO, MnSOD], inflammatory genes [ IL1-beta], metastatic potential [MMP1],
and cell cycle or tumor suppression [p21, p53]) and related path genes of
susceptibility for esophageal cancer.
OUTLINE: Blood and tumor tissue samples are collected. DNA purified from these samples is
analyzed using DNA-based assays to determine polymorphisms in various related gene pathways.
Patients complete questionnaires concerning environmental, smoking and diet habits.
PROJECTED ACCRUAL: A total of 1,000 tissue samples from patients and healthy participants
(750 patients and 250 healthy participants) will be accrued for this study.
Polymorphisms in various pathways, DNA repair, free-radical formation, inflammatory genes, metastatic potential, and cell cycle or tumor suppression in blood samples
David C. Christiani, MD
Massachusetts General Hospital
|Massachusetts General Hospital||Boston, Massachusetts 02114-2617|
|Harvard School of Public Health||Boston, Massachusetts 02115|