Identification of Genomic Lesions Promoting Nodal Metastasis in Malignant Melanoma
- Determine the genetic profile of primary melanomas with and without synchronous
regional nodal involvement by examining for 1) activating mutations B-Raf and N-Ras
associated with melanoma development, and 2) allelic imbalances across the genome.
- Compare the genetic profile of primary melanomas from patients with and without lymph
- Determine the combinations of genetic lesions that correlate with nodal metastasis by
adopting a statistical machine learning approach to build a lesion-based classifier for
OUTLINE: Laser capture microdissection is performed on the archived tissue samples to
isolate melanoma cells. DNA is then purified from the samples and amplified using PCR.
Matrix-assisted laser desorption/ionization (MALDI)-time of flight mass spectrometry
technology is used to detect mutations of B-Raf and N-Ras. Single nucleotide polymorphism
arrays are also performed.
Information about the patient's demographics (e.g., TNM staging, sex, age, and tissue
collection dates) will be gathered by chart review or from the Multidisciplinary Melanoma
Conference at University Hospitals tumor conference report in order to match cases.
Time Perspective: Prospective
Genetic profile of patients with primary melanomas with and without synchronous regional nodal involvement
at the time of presentation
Henry Koon, MD
Case Medical Center, University Hospitals Seidman Cancer Center, Case Comprehensive Cancer Center
United States: Institutional Review Board
|Case Medical Center, University Hospitals Seidman Cancer Center, Case Comprehensive Cancer Center||Cleveland, Ohio 44106-5065|