Inclusion Criteria

- INCLUSION CRITERIA:

Known HIV disease and followed in the NCI pediatric HIV program (on an NCI protocol) as of
December 2004, or an HIV-infected sibling of a participant.

Age greater than 6 years.

For children greater than 7 years, ability to give assent if developmentally appropriate.

Receives care from a health care provider not affiliated with the protocol.

INCLUSION CRITERIA FOR CARDIAC IMAGING SUB-STUDY:

HIV positive participants

- Known HIV disease, documented or believed to have been acquired during the first
decade of life

- Age greater than 7 years old

HIV negative participants

- HIV negative, documented by a negative ELISA

- Age greater than or equal to 18 years old and less than or equal to 30 years old

EXCLUSION CRITERIA:

Inability or unwillingness to provide informed consent for subjects greater than or equal
to 18 years, and inability or unwillingness of one parent/legal guardian to provide
consent for subjects less than 18 years.

Clinically significant, systemic illness (serious infections or significant cardiac,
pulmonary, hepatic or other organ dysfunction) which in the judgment of the Principal
Investigator would compromise the patient's ability to tolerate this study.

EXCLUSION CRITERIA FOR CARDIAC IMAGING SUB-STUDY:

Subjects with contraindication to MRI scanning. These include but are not limited to the
presence of any implanted device that is incompatible with MRI.

Subjects who cannot tolerate an MRI scan or who require sedation for MRI.

Pregnant or lactating women.

History of severe allergic reaction to gadolinium contrast agents.

Estimated glomerular filtration rate less than 60 cc/min/1.73m(2).

Inability or unwillingness to provide informed consent for subjects greater than 18 years,
and inability or unwillingness of one parent/legal guardian to provide permission for
subjects less than 18 years.

Clinically significant, systemic illness (serious infections or significant cardiac,
pulmonary, hepatic or other organ dysfunction) which in the judgement of the Principanl
Investigator (or designee) would compromise the patient's ability to tolerate this study

Some subjects may be excluded from cardiac CT but still eligible to enroll in the rest of
the study. Exclusion criteria for the cardiac CT component include:

- Age less than 18 years

- Use of metformin-containing products less than 24 hours prior to contrast
administration

- History of Multiple Myeloma

Some subjects may be excluded from receiving contrast but will have a non-contrast cardiac
CT. Exclusion from receiving contrast include:

- Cratinine value greater than 1.4 mg/dL

- History of significant allergic reaction to CT contrast agents

Some subjects may receive the cardiac CT but will be excluded from receiving beta-blocker.
Exclusion criteria for receiving beta-blocker include:

- Asthma

- Active bronchospasm

- Moderate or severe COPD

- 2nd or 3rd degree AV block

- Decompensated cardiac failure

- Allergy to beta blockers

- Systolic blood pressure less than 100 mm HG

- Resting heart rate at the time of scan less than 60 if regular and less than 65 if
irregular