Reproducibility of Serum Sex Steroid Measurement: A Proof of Performance Study
- Evaluate reproducibility of sex steroid assays, including testosterone,
dihydrotestosterone, androstenedione, estrone, estradiol, androstenediol, and
dehydroepiandrosterone (DHEA) by gas chromatography-mass spectrometry (GC-MS) and
androstenediol glucuronide, androsterone glucuronide, DHEA sulfate, and estrone sulfate
by liquid chromatography (LC)-MS-MS.
- Evaluate the reproducibility of the measurement of these steroids and metabolites by
- Compare the assay results of GC-MS and LC-MS-MS to those of RIA.
OUTLINE: Participants undergo blood collection for laboratory analysis. Samples are analyzed
by gas chromatography-mass spectrometry (MS), liquid chromatography-MS-MS, and
radioimmunoassay to assess the reproducibility of these assays for measuring sex steroids.
Reproducibility of sex steroid assays including gas chromatography-mass spectrometry (GC-MS) and liquid chromatography (LC) -MS-MS
Ann Hsing, MD
National Cancer Institute (NCI)
United States: Federal Government