Investigating Markers of Radiation Outcome in Patients With Intermediate-Risk Prostate Cancer Using DNA Microarray Analysis: An RTOG Pilot Study
- Demonstrate the feasibility of acquiring specimens, both frozen and paraffin-embedded,
to yield RNA of adequate quality to perform DNA microarray studies on markers of
radiotherapy outcome in patients with intermediate-risk prostate cancer.
- Compare the gene expression results from frozen tissue and paraffin-embedded tissue to
see the correlation between the 2 methods in patients treated on the brachytherapy
alone arm of protocol RTOG-0232.
- Find genes that are expressed differentially before and after radiation therapy in
patients treated on the brachytherapy plus external-beam radiation therapy arm of
- Find gene expression patterns in patients treated on either arm of protocol RTOG-0232
who have significant associations with biochemically as well as clinically apparent
local and distant failure.
OUTLINE: This is a multicenter, pilot study.
Frozen and paraffin-embedded tissue samples are studied for biomarker/laboratory analysis.
DNA microarray analysis is performed on the samples to assess markers of radiotherapy
PROJECTED ACCRUAL: A total of 156 patients will be accrued for this study.
Number of frozen and paraffin-embedded specimens that yield adequate RNA for tumor tissue microarray analysis
Arnab Chakravarti, MD
Ohio State University
United States: Federal Government
|Winship Cancer Institute of Emory University||Atlanta, Georgia 30322|
|Siteman Cancer Center at Barnes-Jewish Hospital - Saint Louis||St. Louis, Missouri 63110|
|Fox Chase Cancer Center - Philadelphia||Philadelphia, Pennsylvania 19111-2497|
|Arthur G. James Cancer Hospital and Richard J. Solove Research Institute at Ohio State University Comprehensive Cancer Center||Columbus, Ohio 43210-1240|
|MedCentral - Mansfield Hospital||Mansfield, Ohio 44903|
|Cancer Institute at St. John's Hospital||Springfield, Illinois 62701|
|Georgia Cancer Center for Excellence at Grady Memorial Hospital||Atlanta, Georgia 30303|
|Emory Crawford Long Hospital||Atlanta, Georgia 30308|
|Barnes-Jewish West County Hospital||Saint Louis, Missouri 63141|
|Siteman Cancer Center at Barnes-Jewish St. Peters Hospital - St. Peters||Saint Peters, Missouri 63376|