Detection and Quantification of Changes in Levels of Protein Expression and Phosphorylation Status Using Human Hair Samples
- Determine whether layered membrane technology can quantitatively measure the
expression, activity, and localization of cell growth control and signaling proteins in
hair samples from patients with cancer.
- Correlate changes in protein expression with expected changes based on planned therapy
in these patients.
OUTLINE: This is a pilot, multicenter study.
Hair samples are obtained from patients at baseline and at the end of study. The samples are
assayed for proteins and phosphorylation status using layered membrane technology. Proteins
analyzed may include vascular endothelial growth factor receptor (VEGFR), Erk1/2, Akt,
epidermal growth factor receptor (EGFR), and c-KIT.
PROJECTED ACCRUAL: A total of 190 patients will be accrued for this study within 3 years.
Obtain data on the detection and quantification of changes in levels of protein expression and phosphorylation status using human hair samples at baseline and after completion of study
Steven K. Libutti, MD
NCI - Surgery Branch
United States: Federal Government