Gene Expression Profiling of Metastatic Colon Cancer (CCCWFU 89B03)
- Evaluate gene expression profiles in normal and tumor tissue from patients with colon
cancer metastatic to the liver, lungs, or peritoneum.
- Establish cell lines from primary colon tumors metastatic to the liver, lungs, or
- Determine the specific gene expression changes that result in the manifestation of the
drug-resistant phenotype for each metastatic site.
OUTLINE: This is a pilot study.
Tumor and normal tissue collected during surgery are analyzed for gene expression profiling
by cDNA microarray. Tissue is also analyzed for thymidylate synthase (TS) gene expression by
quantitative PCR and for protein expression by western blot. Gene expression patterns are
correlated with TS levels.
Observational Model: Cohort, Time Perspective: Retrospective
Gene expression changes that occur at each metastatic site (i.e., liver, lungs, and peritoneum)
Perry Shen, MD
Comprehensive Cancer Center of Wake Forest University
United States: Institutional Review Board