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A Phase 1, Open Label, Dose-escalation, Pharmacodynamic Study of Intranodal Injection of Adenovirus-CD154 (Ad-ISF35) in Patients With Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma

Phase 1
18 Years
Not Enrolling
Chronic Lymphocytic Leukemia, Small Lymphocytic Lymphoma

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Trial Information

A Phase 1, Open Label, Dose-escalation, Pharmacodynamic Study of Intranodal Injection of Adenovirus-CD154 (Ad-ISF35) in Patients With Chronic Lymphocytic Leukemia/Small Lymphocytic Lymphoma

Inclusion Criteria:

1. Diagnosis of B-cell CLL/SLL including

- Lymphocytosis of monoclonal B-cells co-expressing ≥ one B-cell marker (CD19,
CD20, or CD23) and CD5 in peripheral blood or lymph node AND

- Bone marrow with ≥ 30% mononuclear cells having the CLL/SLL phenotype.

2. Presence of at least ONE single accessible AND palpable lymph node in the cervical,
supraclavicular, axillary, or inguinal regions. The size of the lymph nodes must be
larger than 2x2 cm in the horizontal and perpendicular axes.

3. Intermediate or High risk, poor prognosis CLL/SLL

4. Indication for treatment as defined by the NCI Working Group Guidelines:

- Massive (i.e. > 6 cm below the left costal margin) or progressive splenomegaly

- Massive lymph nodes or nodal clusters (i.e. > 10 cm in longest diameter), or
progressive lymphadenopathy OR

- Grade 2 or 3 fatigue OR

- Fever ≥ 100.5˚F or night sweats for greater than 2 weeks without documented
infection OR

- Presence of weight loss ≥ 10% over the preceding 6 months OR

- Progressive lymphocytosis with an increase of ≥ 50% over a 2-month period or an
anticipated doubling time of less than 6 months.

- Evidence of progressive marrow failure as manifested by the development of, or
worsening of, anemia and / or thrombocytopenia.

- Autoimmune anemia and / or thrombocytopenia poorly responsive to corticosteroid

5. Males and females 18 years of age and older

6. Laboratory parameters as specified below:

- Hematologic: Hemoglobin ≥ 10 g/dL (may be post-transfusion); platelet count ≥ 50

- Hepatic: Total Bilirubin < 2 X ULN, and ALT and AST < 2 x ULN

- Renal: Creatinine ≤ 2 X ULN

7. ECOG Performance Status ≤ 2

8. Anticipated survival of at least 3 months

9. For men and women of child-producing potential, use of effective barrier
contraceptive methods during the study and for one month following treatment.

10. Ability to understand the requirements of the study, provide written informed consent
and authorization of use and disclosure of protected health information, and agree to
abide by the study restrictions and return for the required assessments.

11. Negative test results for current/active infection with HIV-1, HIV-2, HTLV-1, HTLV-2,
hepatitis A, B, C within 30 days of registration. (Antibody, antigen and nucleic acid
tests acceptable, depending on institutional standards.).

Exclusion Criteria:

1. Pregnant or nursing women

2. Treatment with chemotherapy or monoclonal antibody within 28 days prior to entering
the study.

3. Treatment with chemotherapy or monoclonal antibody during the time of participation
in this trial.

4. Grade 3 or 4 cardiac disease as defined by the New York Heart Association Functional

5. Severe or debilitating pulmonary disease (dyspnea at rest, significant shortness of
breath, COPD)

6. Participation in any investigational drug study within 28 days prior to ISF35
administration. (Patient must have recovered from all acute effects of previously
administered investigational agents)

7. History of malignancy other than CLL within five years of registration, except
patients with adequately treated basal, squamous cell carcinoma or localized cervical

8. Active symptomatic fungal, bacterial and/or viral infection including active HIV or
viral (A, B or C) hepatitis.

9. Any illness or condition that in the opinion of the Investigator may affect safety of
treatment or evaluation of any the study's endpoints.

Type of Study:


Study Design:

Allocation: Non-Randomized, Intervention Model: Single Group Assignment, Masking: Open Label, Primary Purpose: Treatment

Outcome Measure:

Determine the safety and maximum tolerated dose (MTD) of Adenovirus-CD154 (Ad-ISF35) when injected directly into lymph nodes of patients with CLL/SLL.

Outcome Time Frame:

2 years

Safety Issue:


Principal Investigator

Januario Castro, M.D.

Investigator Role:

Principal Investigator

Investigator Affiliation:

Assistant Clinical Professor in the Blood and Marrow Transplantation Division


United States: Food and Drug Administration

Study ID:




Start Date:

February 2006

Completion Date:

October 2009

Related Keywords:

  • Chronic Lymphocytic Leukemia
  • Small Lymphocytic Lymphoma
  • Leukemia
  • Leukemia, Lymphocytic, Chronic, B-Cell
  • Leukemia, Lymphoid
  • Lymphoma



University of California, San Diego Moores Cancer Center San Diego, California  92093