Trial Information

Clinical Application of Autologous Three-Cellular Cultured Skin Substitutes (CSS) Based on Esterified Hyaluronic Acid Scaffold: Our Experience

Background and Objective:

Permanent wound closure remains a limiting factor in the closure of extensive,
full-thickness loss of substance. The aim of this work was to present our experience in the
use of autologous three-cellular cultured skin substitutes (CSS) consisting of cultured
keratinocytes, melanocytes and fibroblasts attached to a scaffold of hyaluronic acid. This
method represents a surgical alternative in the treatment of a variety of pathologies,
including burns, ulcers and giant nevi.

Methods:

CSS were obtained from full-thickness skin biopsies collected after enrollment of 11
patients in a study protocol approved by the local Institutional Review Boards of the "La
Sapienza" University of Rome. CSS consisted of a structure characterized by the presence of
a pluristratified epithelial cell surface with melanocytes (relation 1/20) and a basement of
fibroblasts kept together by an esterified hyaluronic acid scaffold that can be surgically
manipulated, and is gradually reabsorbed after implantation and substituted by the host
connectival stroma.