A Human Intervention Trial Studying Gene Expression in High-Grade Prostatic Intraepithelial Neoplasia Following Consumption of Broccoli or Peas
Cancer is one of the main causes of death among humans in the world. Prostate cancer affects
20,000 men in the UK alone each year. Diet is known to be a major factor that influences
risk of cancer. Therefore, changes to the diet may alter cancer risk. Cruciferous
vegetables, particularly broccoli, provide the diet with a substantial source of plant
chemicals called glucosinolates. Previous research has indicated that glucosinolates break
down to form isothiocyanates (ITCs) that can be absorbed in the body. Further research has
indicated that ITCs may protect the body against the development of prostate cancer.
However, the mechanisms behind this effect are not fully understood.
ITCs are thought to be powerful anti cancer agents as they can modulate the expression
(switching on or off) of specific genes involved in the removal of toxic substances such as
carcinogens from the body. In this study we wish to evaluate the effect of consumption of a
conventionally bred cultivar of broccoli containing high levels of ITCs on gene expression
in prostate tissue to gain a better understanding of its mechanism of action. Several
studies suggest a further protection against cancer amongst individuals who have a deletion
of certain genes.
This pilot study comprises a 12-month intervention of either 400g ITC-enriched broccoli per
week of 400g garden peas in men at high risk of developing prostate cancer. Changes in gene
expression of prostate biopsy tissue will be compared before and after 6 and 12 months of
intervention in both dietary groups.
Allocation: Non-Randomized, Endpoint Classification: Pharmacokinetics Study, Intervention Model: Parallel Assignment, Masking: Open Label, Primary Purpose: Basic Science
Changes in gene expression in RNA extracted from prostate tissue
Baseline, 6 months and 12 months
Richard F Mithen, PhD
Institute of Food Research, Norwich
Norwich Research Ethics Committee UK: