Identification of Differentially Expressed Genes in Hepatocellular Carcinoma With DNA Microarrays
The clinical samples will be collected in the NTUH, while the DNA microarray works will be
done in Biomedical Engineering Center (BMEC) of ITRI.
Patients A total of 40 cases of HCC patients (20 HBV-related HCC and 20 HCV-related HCC) who
received surgical resection in National Taiwan University Hospital will be enrolled from our
tissue banks. The diagnosis of HCC was confirmed by pathological examination. The surgical
specimens were frozen immediately after surgery and stored in –140°C. The
clinicopathological data will be retrospectively obtained from chart reviews.
Another total of 40 new HCC patients who receive surgical resection in National Taiwan
University Hospital will be collected. The healthy subjects and patients chronically
infected with HBV or HCV without HCC will be used as controls. The whole bloods will be
collected before, one and six months after operation. The diagnosis of HCC will be confirmed
by pathology. In addition to the whole bloods, the surgical specimens will be frozen
immediately after surgery and stored in –140°C.
Observational Model: Defined Population, Primary Purpose: Screening, Time Perspective: Longitudinal, Time Perspective: Retrospective/Prospective
Chien-Hung Chen, MD
National Taiwan University Hospital
Taiwan: Department of Health