Eutopic endometrium was obtained and separated into single endometrial stromal cell (ESC) in
women with adenomyosis (study group) and without adenomyosis (control group).
After becoming pre-confluent (covering 80% of the culture well), ESC was cultured for 8 days
solely or with the addition of medroxyprogesterone (MPA) or danazol.
ELISA was done to measure IL-6, IL-8, and TNF-alpha concentrations of the culture media.
Real-time quantitative RT-PCR was done to measure IL-6, IL-8, and TNF-alpha RNA levels in
Observational Model: Defined Population, Observational Model: Natural History, Time Perspective: Cross-Sectional, Time Perspective: Prospective
Jehn-Hsiahn Yang, M.D.
Department of Obstetrics and Gynecology, National Taiwan University Hospital
Taiwan: Department of Health