Identification of Biomarkers Associated With Human Hepatocellular Carcinoma by PROTEOMEX
Cell lines Hep3B and Huh7 HCC cell lines will be cultured in (Dulbecco’s modified Eagle
medium) DMEM containing 10% fetal calf serum and incubated at 37 C in 5% CO2 incubator. The
rationale of using HCC cell line instead of clinical HCC samples is that HCC tissues are
heterogeneous and might contain some non-HCC cells. The cell will be harvested when cells
reach 95% confluence.
Sera collection 20 mL of venous 20 mL of venous blood was collected from 20 HBV-related HCC
and 20 HCV-related HCC patients before treatment. The venous blood was allowed to clot,
centrifuged at 800 g for 5 min, and serum was aliquoted and stored at –80 0C. Control sera
were obtained from 20 HBV carriers without HCC, 20 chronic hepatitis C patients without HCC,
and 10 normal subjects without liver diseases.
Two-dimensional gel electrophoresis using patients' sera for immunoblotting will be done.
Spots of interest will be identified by delayed extraction matrix-assisted laser desorption
Observational Model: Defined Population, Primary Purpose: Screening, Time Perspective: Cross-Sectional, Time Perspective: Retrospective/Prospective
Chien-Hung Chen, MD
National Taiwan University Hospital
Taiwan: Department of Health