Trial Information

Identification of Biomarkers Associated With Human Hepatocellular Carcinoma by PROTEOMEX

Cell lines Hep3B and Huh7 HCC cell lines will be cultured in (Dulbecco’s modified Eagle
medium) DMEM containing 10% fetal calf serum and incubated at 37 C in 5% CO2 incubator. The
rationale of using HCC cell line instead of clinical HCC samples is that HCC tissues are
heterogeneous and might contain some non-HCC cells. The cell will be harvested when cells
reach 95% confluence.

Sera collection 20 mL of venous 20 mL of venous blood was collected from 20 HBV-related HCC
and 20 HCV-related HCC patients before treatment. The venous blood was allowed to clot,
centrifuged at 800 g for 5 min, and serum was aliquoted and stored at –80 0C. Control sera
were obtained from 20 HBV carriers without HCC, 20 chronic hepatitis C patients without HCC,
and 10 normal subjects without liver diseases.

Two-dimensional gel electrophoresis using patients' sera for immunoblotting will be done.
Spots of interest will be identified by delayed extraction matrix-assisted laser desorption
ionization.