Gene Expression Profiling and Immunological Mechanism Affects the Response of Malignant Cavity Effusion Towards DC-CIK Immunotherapy
1. The patients with malignant cavity effusion are treated with dendritic cells (DC) plus
cytokine induced killer cells (CIK) locally.
2. Malignant cavity effusion from cancer patients is obtained through puncture and is
centrifugalized to get supernatant fluid and enrich cancer cells before and after the
3. The enriched cancer cells which are flash frozen, as well as the supernatant, are
stored at -80°C until processing.
4. The T-Cell Receptor/B-Cell Receptor gene expression in cavity effusion is detected by
micro-array to explore the mechanism that DC-CIK immunotherapy controls the malignant
5. Statistical analysis is performed using unsupervised hierarchical cluster.
Observational Model: Cohort, Time Perspective: Prospective
T-Cell Receptor and B-Cell Receptor gene expression profiling and the change of cytokines, lymphocytes subpopulation before and after DC-CIK infusion
Jun Ren, MD, PhD
Capital Medical University Cancer Center /Beijing Shijitan Hospital
China: Ethics Committee