The Effect of Human Placental Explants and Pregnant Women Sera on Cancer Cells
In the Oncogenetic laboratory cancer cells will be exposed to first trimester human
placental explants or to serum collected from pregnant women. As a control cancer cells will
be cultured alone or in the presence of sera collected from young (18-45) non pregnant
Placentae, 6-9 weeks gestational age, will be retrieved from terminated normal pregnancies.
The placenta will be taken after pregnancy termination, thus, no harm will be caused for the
The sera will be collected from the women by an authorized physician. It will be taken from:
1. the vein of the non pregnant women and volunteered pregnant women. 2. from the same
branula that will be introduced into the pregnant women as a routine during pregnancy
Following culture we will analyze the cancer cells phenotype (cell death, proliferation,
migration, and invasion), signaling pathway, mRNA, miRNA and protein expression.
Sera will be collected from 50 pregnant women and 50 non pregnant women. Placentae will be
collected from 50 pregnant women.
Cancer cell phenotype (in the laboratory)
Changes in: cell death (% from tested cells) cell cycle (% cells in G1, G2 and S phases) cell migration (% closure in Scratch test) cell invasion (% cells passing through membrane in Transwell assay) RNA repertoire (Fold change) miRNA repertoire (Fold change)
Michael Lishner, MD
Meor Medical Center, Kfar-Saba, Israel
Israel: Ministry of Health