Effects of NAC Supplementation on Skeletal Muscle Performance Following Aseptic Injury Induced by Exercise
The major thiol-disulfide couple of reduced (GSH) and oxidized glutathione (GSSG) is a
key-regulator of major transcriptional pathways regulating aseptic inflammation and recovery
of skeletal muscle following aseptic injury. Antioxidant supplementation may hamper
exercise-induced cellular adaptations.
Our objective was to examine how thiol-based antioxidant supplementation affects skeletal
muscle's performance and redox-sensitive signalling during the inflammatory and repair
phases associated with exercise-induced micro-trauma.In a double-blind, counterbalanced
design, 12 men received placebo (PLA) or N-acetylcysteine (NAC, 20 mg/kg/day) following
muscle-damaging exercise (300 eccentric contractions). In each trial, muscle performance was
measured at baseline, post-exercise, 2h post-exercise and daily for 8 consecutive days.
Muscle biopsies from vastus lateralis and blood samples were collected pre-exercise and 2h,
2d, and 8d post-exercise.
Intervention Model: Single Group Assignment, Masking: Double Blind (Subject, Outcomes Assessor), Primary Purpose: Basic Science
Change in reduced glutathione in blood
Concentration of reduced glutathione in red blood cells
one hour before exercise, 5 minutes post-exercise, 2 hours post-exercise, daily for 8 days post-exercise
Ioannis F Fatouros, Ph.D.
Democritus University of Thrace, Greece
Greece: Institutional Review Board