Trial Information

Staging and Outcome Depending on Surgical Treatment in Adenocarcinomas of the Oesophagogastric Junction

A prospective database of all patients with surgically resectable esophageal, cardia and
gastric carcinomas was established in 1992 at the Department for General, Visceral and
Thoracic Surgery at the University Hospital Hamburg-Eppendorf, Germany. Only patients
without neoadjuvant therapy and histologically proven AEG type I and II were included into
this study. The demographic, clinical, operative and postoperative courses of each patient
were collected. Informed consent was obtained from all patients before including them in the
prospective database which was approved by the Medical Ethical Committee of the chamber of
physicians of Hamburg.

Preoperative examinations of patients included physical examination, routine blood tests,
plain chest radiography, abdominal ultrasonography, endoscopy, endosonography (EUS),
computed tomography (CT) of the chest and abdomen as well as positron emission tomography
(PET)-CT after January 2006 and studies of tumor markers (CEA, CA 19-9), in selected
patients. Tumour localization was defined according the Siewert classification (3) based
upon endoscopy, endosonography and intraoperative assessment of the respective surgeon.

All patients undergoing a thoracoabdominal esophagectomy (TA) were operated with a
right-sided thoracotomy, and a median inverse T-shaped laparotomy. Peritumoral resection
included an en bloc subtotal esophageal resection with dissection of the right-sided
paratracheal, aortopulmonary window, subcarinal and mediastinal lymph nodes (LN) and the
azygos vein and a collar or high intrathoracically anastomosis. An extensive lymphadenectomy
of the upper abdominal compartment (D-II lymphadenectomy, including the paracardial, the
left gastric artery nodes along with the LN of the lesser and greater curvature of the
stomach, the prepyloric LN, the celiac trunk, the common hepatic artery, the LN in the
hepathoduodenal ligament and the LN at the splenic artery) was conducted.

The transhiatal esophagectomy (TH) consisted of an inversed T-shaped laparotomy, a wide
peritumoral dissection of the distal esophagus, DII lymphadenectomy and a dissection of the
LN of the posterior mediastinum extending as far as the carina of the trachea.

Extended gastrectomy (EG) consisted of an inversed T-shaped laparotomy, a gastrectomy with
resection of the distal esophagus, D-II lymphadenectomy plus dissection of the lower
posterior mediastinum LN. The reconstruction included a jejunal loop with a J-pouch.

Histopathological analyses were performed by a senior specialist in gastrointestinal
pathology. All removed LN were counted, identified according to their location and assessed
separately. Standard histopathologic analysis of paraffin-embedded LN was performed by
serial sections of 5 µm thickness and staining with hematoxylin-eosin and van Gieson.

The histopathological report including tumour type, stage and grade was determined according
to the 7th edition of the TNM classification (8, 12). The percentage of positive lymph nodes
in regard to excised lymph nodes was classified according the definition of Bogoevski et al.
(13).

Postoperative follow-up was conducted at three months intervals for the first two years and
at six-month intervals thereafter, and included physical examination, plain chest
radiography, abdominal ultrasonography, endoscopy, endosonography, computed tomography of
the chest and abdomen as well as PET-CT after January 2006 in selected patients. Further,
studies of tumor markers (carcinoembryonic antigen and CA 19-9), and bone scanning were
performed. Recurrence was diagnosed if proven by biopsy or unequivocal evidence of tumour
masses with a tendency to grow during further follow-up and/or follow-up until death. Events
considered were death, local recurrence, and distant metastasis. When no events were
recorded, the patients were censored for the statistical evaluation at the last contact.
Overall survival, defined as time from operation to death or last follow-up, and local
recurrence-free survival, defined as time from operation to time of diagnosis of local
tumour recurrence, were calculated.

Statistical analysis SPSS 17.0 for Windows was used for statistical analysis. Associations
between categorical variables were assessed by χ2 test. The Mann-Whithney U and
Kruskal-Wallis tests were used for analysis of continuous variables. The Kaplan-Meier method
was used to analyse recurrence and death. Differences between patient groups were assessed
using log-rank tests. Apart from patient's sex and age at surgery, those co-variates with a
p-value <0.05 in univariable survival analysis (log rank test) were entered into
multivariable Cox proportional-hazards analysis to assess the independent influence of these
co-variates and shown as hazard ratio (HR) and 95 per cent c.i.. Because this analysis was
intended to be explorative, no adjustment for multiple testing was carried out. Differences
were considered to be statistically significant at P<0.05.