Histopathologic Evaluation of High Grade Brain Tumors by High Order Diffusion Tensor Imaging: Peritumoral Glial Cell Infiltration Quantitative Method
Patients with high grade brain tumor undergo a usual brain tumor MRI protocol as well as a
20 or 60 directions DT-MRI sequence upon patient collaboration. Images are acquired using a
3 Tesla MRI scanner (GE MR750 Discovery).
The usual brain tumor MRI protocol contains the following sequences: 3DT1, Axial T2, Flair,
T2 EG, diffusion (b0-b1000), 3DT1CE.
They will then undergo stereotactic tumor biopsies with Leksell frame mounted.
Biopsy targets will be surgically planned. The coordinates of this first biopsy will be
recorded for later coregistration with DT-MRI datasets.
Histopathologic analysis of the biopsies will be done using usual procedure. various
immunologic markers such as MAP2, Ki67 will be used on the first biopsy sample in order to
estimate the percentage of infiltration (ratio of tumor cells number over total cells
Analysis of DT-MRI datasets will be done using home-made software for high order tensor
resolution and GA estimation at the exact coordinates of the biopsy sites.
Finally a statistical analysis (Pearson or Spearman) will be done to correlate GA and the
Intervention Model: Single Group Assignment, Masking: Single Blind (Subject), Primary Purpose: Screening
University Hospital, Clermont-Ferrand
France: Ministry of Health