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Enumeration and Function Analysis of Treg Cells in Peripheral Blood of HCC Patients Before and After Ablation Therapy


N/A
18 Years
80 Years
Open (Enrolling)
Both
Hepatocellular Carcinoma

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Trial Information

Enumeration and Function Analysis of Treg Cells in Peripheral Blood of HCC Patients Before and After Ablation Therapy


Regulatory T cells，which are also called Treg cells，play an important role in suppressing
anti-tumor immunity.Accumulated evidences indicate that Treg cells are elevated in
peripheral blood,however,there are also reports that decreased Treg cells are found in
hepatocellular patients.This study focuses on the changes of Treg cells ratio in peripheral
blood of hepatocellular carcinoma patients before and 1 week, 4 weeks after ablation ,also
its functional cytokines,such as TGF-β,IL-10,IFN-γ,and inhibition function when co-cultured
with CD4+CD25-、CD8+ cells. The investigators speculate that a decreased Treg ratio will be
found in patients who receive ablation therapy,and its function
cytokines,moreover,CD4+CD25-、CD8+ cells' proliferation and function could be inhibited when
co-cultured with Treg cells.


Inclusion Criteria:



- hepatocellular patients diagnosed through biopsy;or either dynamic imagine with a
diagnosis of hepatocellular carcinoma and alphafetoprotein＞400μg/L；or two or more
dynamic imagine with a diagnosis of hepatocellular carcinoma

- Child-Pugh A or B

- well preserved renal and hematopoietic Function

- receive ablation therapy through percutaneous radiofrequency ablation or microwave
ablation or ethanol injection ablation or any kind of combination of them.

- achieve complete ablation accessed by contrast-enhanced CT

Exclusion Criteria:

- incomplete ablation

- remote metastasis

- Child-Pugh C

- general infection

- autoimmune diseases

- suffer from other tumors concurrently or in last five years

- patients with immune deficit or infected by HIV

- receiving glucocorticoid or other medicine inhibiting immune system

Type of Study:

Observational

Study Design:

Observational Model: Case-Only, Time Perspective: Prospective

Outcome Measure:

variation of absolute counting of T cell subsets in peripheral blood of HCC patients before and 1,4 weeks after radiofrequency ablation therapy

Outcome Description:

changes of absolute counting of T cell subsets in peripheral blood of HCC patients before and 1,4 weeks after radiofrequency ablation therapy,such as CD3+,CD4+,CD8+,CD4+CD25+FOXP3+(Treg) cells.

Outcome Time Frame:

4 weeks post therapy

Safety Issue:

No

Principal Investigator

Ming Kuang, MD,PhD

Investigator Role:

Study Chair

Investigator Affiliation:

First Affiliated Hospital, Sun Yat-Sen University

Authority:

China: Ministry of Science and Technology

Study ID:

2010B031600209

NCT ID:

NCT01668381

Start Date:

August 2012

Completion Date:

December 2013

Related Keywords:

  • Hepatocellular Carcinoma
  • hepatocellular carcinoma
  • Catheter Ablation
  • T-Lymphocytes, Regulatory
  • IL-10
  • TGF-β
  • IFN-γ
  • Carcinoma
  • Carcinoma, Hepatocellular

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