Trial Information

Genomic Analysis of Adolescent and Young Adult Acute Lymphoblastic Leukemia

OBJECTIVES:

- To identify somatically acquired genetic copy number and sequence alterations at the
time of diagnosis in adolescent and young adults (AYA) acute lymphoblastic leukemia
(ALL) samples and to correlate them with clinical and laboratory characteristics and
outcome.

- To identify specific microarray multi-gene and multi-exon expression signatures at the
time of diagnosis and to correlate them with clinical and laboratory characteristics
and outcome.

- To gain insights into the genetic events that contribute to the formation, development
and relapse of AYA ALL by integrating the copy number and sequence alterations with the
multi-gene signatures and by comparing these with data already generated in pediatric
ALL.

OUTLINE: Cryopreserved samples are analyzed for DNA copy number alterations and
loss-of-heterozygosity, gene expression profiling, and mutation analysis by single
nucleotide polymorphism (SNP) microarrays, Affymetrix Exon arrays, and whole genome
amplification (WGA, Repli-G Qiagen). Confirmation studies are then done by fluorescence in
situ hybridization (FISH), reverse transcriptase (RT)-polymerase chain reaction (PCR), and
rapid amplification of cDNA ends (RACE).