Rapid Identification of Leukemia Stem Cells Associated With AML1-ETO and Inv(16) Through Characterization of Oncogene-Induced Changes in Cell-Surface Antigen Profiles on Hematopoietic Stem Cells
- To address whether the mutation-specific cell-surface markers observed in murine system
will allow the prospective isolation of leukemia stem cells (LSC) from human bone
marrow samples that have the same cytogenetic abnormalities.
- To compare the incidence of leukemia in NSG mice that have received CD34+CD38 marker+
cells to NSG mice that receive what are hypothesized to be normal cells (CD34+CD38
marker-subset) from the same patient.
OUTLINE: Samples and controls are sorted and re-sorted for CD34, CD38, and CD55 subsets by
single-cell polymerase chain reaction (PCR) analysis, flow cytometry, and
reverse-transcriptase PCR. Sorted cell subsets are then transplanted into NSG mice.
Beginning 6 weeks after transplantation, peripheral blood samples are collected and analyzed
for human lymphoid- and myeloid-lineage cells by fluorescence-activated cell sorting (FACS).
Identification of LSC subset in a NSG transplantation assay
Stephanie C. Heidemann, MD
University of Alabama at Birmingham
United States: Federal Government