A Prospective Comparison of Transcriptional Profiling of Luteal Phase Endometrial Biopsies After Induction of Oocyte Maturation With a Gonadotropin Releasing Hormone (GnRH) Agonist or Human Chorionic Gonadotropins (hCG)
Ovarian hyperstimulation syndrome (OHSS) is an iatrogenic complication of controlled ovarian
hyperstimulation (COH) which may result in significant morbidity and rarely mortality as
well as significant financial and psychological distress. The use of a GnRH agonist for
induction of final oocyte maturation in ovarian stimulation cycles utilizing GnRH antagonist
for pituitary suppression has proven to be an effective method of preventing the risk of
Unfortunately, some studies, but not all, have also reported lower pregnancy rates in these
cycles as compared to cycles using hCG trigger and this has been attributed to possible
impaired endometrial receptivity.
The investigators intend to obtain endometrial biopsies collected from the same subject in a
natural cycle and then a biopsy during either a GnRH agonist or hCG triggered stimulation.
Expression profiles of mRNAs will first be screened using microarray technology. Relative
levels of specific mRNAs that display altered expression in the GnRH-triggered samples, as
assayed by microarray, will then be confirmed by real-time, quantitative reverse
transcription/polymerase chain reaction (Q-PCR). The investigators shall also evaluate
patients feeling of well being and physical quality of life after GnRH agonist trigger
compared with hCG trigger.
Allocation: Randomized, Intervention Model: Parallel Assignment, Masking: Single Blind (Investigator), Primary Purpose: Diagnostic
Endometrial gene expression profile
7 days after trigger of oocyte maturation
Lawrence Engmann, MD, MRCOG
University of Connecticut Health Center
United States: Institutional Review Board
|UCHC Division of Reproductive Endocrinology||Farmington, Connecticut 06030|