Investigating the Frequency of Loss of Imprinting Across a Birth Cohort and the Link DNA Methylation Plays
- What is the frequency of loss of imprinting at birth (in the cord blood and placenta)
in a relatively healthy birth cohort?
- Does deoxyribonucleic acid (DNA) methylation levels at imprinting genes have a direct
association to the gene expression?
OUTLINE: Archived tumor tissue, cord blood, and placenta samples are analyzed for DNA
methylation, single nucleotide polymorphism, and gene expression by polymerase chain
reaction (PCR), pyrosequencing, and quantitative real-time PCR. Information regarding gender
and age of the samples are also collected, if possible.
Frequency of loss of imprinting at birth
Karin Michels, MD, PhD
Dana-Farber/Brigham and Women's Cancer Center
United States: Federal Government