The Role of Stroma-Derived Soluble TßRIII in Neuroblastoma
- Determine whether TβRIII expression and TGF-β signaling decrease in advanced-stage
neuroblastoma (NBL) and whether these changes are confined to the Schwannian stroma.
- Determine whether sTβRIII levels and TGF-β signaling correlate with NBL stage, tumor
stroma content, surface TβRIII expression, and TGF-β signaling.
OUTLINE: Archived paraffin-embedded tissue and plasma samples are analyzed for TβRIII
expression, TGF-β signaling, and SMAD3 expression and phosphorylation by
immunohistochemistry, enzyme-linked immunosorbent assay (ELISA), and other assays. Surface
expression of TβRIII in the neuroblastic and stromal tumor components are correlated with
matched circulating levels of soluble TβRIII. Results are then correlated with each
patient's outcome data, including stage.
Expression of TβRIII in the neuroblastic tumor and stroma of patients with advanced-stage NBL
Gerard C. Blobe, MD, PhD
United States: Federal Government