B-precursor Acute Lymphoblastic Leukemia (ALL): Studying the Mechanisms Behind Dexamethasone and Prednisone Sensitivity in High-Risk Patients
- Identify differences in the effect of dexamethasone (Dex) and prednisone (Pred) on
glucocorticoid receptor (GR) occupancy and gene expression in high-risk (HR) B-cell
precursor (BCP)-acute lymphoblastic leukemia (ALL) samples from patients aged 1-9.
- Identify differences in gene expression and GR occupancy of response elements in HR
BCP-ALL patients > 10 years old in response to both Dex and Pred.
OUTLINE: Archived samples are analyzed for gene expression analysis and genomic location of
glucocorticoid receptor by ChIP sequencing (ChiPseq) and RNA sequencing. Data of gene
expression and ChiPseq data sets are compared by luciferase reporter assay.
Differentially expressed genes from both HR BCP-ALL patients aged 1-9 and patients over 10 years old
Miles A. Pufall, PhD
University of Iowa
United States: Federal Government