Kaohsiung Medical University Hospital
Fine-needle aspiration (FNA) cytology or biopsy of hepatic tumor is frequently applied in
the collection of specimens because it is considered safe, efficacious, accurate and cost
effective. Our previous study from small number of patients showed that specimens obtained
from FNA had potential to be applied for primary culture of cancer cells. This study was to
modified our method for primary culture of both hepatocellular carcinoma cells and
cancer-associated fibroblasts in patients with tumor measuring larger than or equal to 3cm.
All patients received one session of aspiration for the diagnosis of hepatic tumor. The
aspirated specimens were applied for both cytologic and pathologic examinations at first.
After then, 10 mL 0.9% NaCl was aspirated by the same needle connected with the same syringe
and injected into 15 mL sterilized centrifugation tube. If the tube contained visible
specimens, these specimens were sent for primary culture.
Observational Model: Case-Only, Time Perspective: Prospective
growth speed of cultured cells
correlation the growth speed of cells (reach equal or more than 70% growth area of 25 cm2 culture flask) and TNM tumor staging
28 days after plating of cells
Zu Y Lin, MD
Kaohsiung Medical University
Taiwan: Department of Health