Neutrophil Extracellular Traps (NETs) Formation Following Chemotherapy for Pediatric Hematological and Solid Tumors, and Its Relation to Other Neutrophil Functions and the Role of NETs in Antitumor Activity
Neutrophil function, including NETs formation, chemotaxis, superoxide production, hydrogen
peroxide production, and the presence of myeloperoxidase, will be examined in 50 pediatric
patientsundergoing chemotherapy for solid and hematological malignancies. Children with the
following malignancies will be examined: acute lymphoblastic leukemia,acute myelogenous
leukemia,Hodgkin's lymphoma,non-Hodgkin's lymphoma, primary bone
sarcoma,rhabdomyosarcoma,non-rhabdomyosarcoma,neuroblastoma,Wilms' tumor,hepatoblastoma or
hepatocellular carcinoma,germ cell tumors, and hystiocytosis. Also those with brain tumors
such as medulloblastom,low grade glioma,high grade glioma,ependymoma,and embryonal and
pineal region tumors. Data gathered on the patients will include background data (age,
gender, ethnicity) and background diseases, data on current illness (histologic type, grade,
stage, response treatment,infectious episodes), and on the use of ranulocyte-colony
stimulating factor (G-CSF).
The following time points will be examined:
1. At diagnosis, before initiation of chemotherapy.
2. immediately before the 2nd course.
3. After the middle course.
4. A month after the last course.
5. Three months after the last course.
6. In acute myelogenous leukemia and acute lymphoblastic leukemia,time points also include
the middle of the maintenance course and 3 months after the end of maintenance.
An additional blood examination will be used to examine NETs formation against tumor cell
lines and their ability to kill tumor cells.
Observational Model: Cohort, Time Perspective: Prospective
Sivan Achituv, MD
The department of pediatric hemato-oncology, Dana Childrens' Hospital, Tel-Aviv Sourasky Medical Center, Tel-Aviv, Israel
Israel: Ministry of Health