Role of the IL-2 Inducible Tcell Kinase in EBV-HLH and EBV+ Hodgkin's Lymphoma
- Evaluate germline DNA from patients with Epstein-Barr virus positive (EBV+) and Hodgkin
lymphoma (HL) for inducible T-cell kinase (ITK) mutations.
- Examine the effects of ITK mutations on expression of the ITK protein.
- Determine whether ITK mutations correlate with specific clinical or histopathological
features of HL.
OUTLINE: Archived blood and tumor tissue samples are analyzed for germline DNA expression
and inducible T-cell kinase (ITK) mutations by PCR, IHC, flow cytometry, and western
blotting, and EBV-encoded RNA (EBER) using in situ hybridization. Each patient's data, such
as date, sex, age, tumor stage and histology at diagnosis, treatment received, response to
treatment, development of recurrent disease, date of last follow-up, and outcomes are also
Presence of germline ITK mutations
Kim E. Nichols, MD, BA
Children's Hospital of Philadelphia
United States: Federal Government