Analysis of GGAA-Microsatellites in Ewing Sarcoma
- To describe the spectrum of GGAA-microsatellite polymorphisms at specific loci in
genomic DNA prepared from Ewing sarcoma tumor specimens.
- To determine if there are differences in GGAA-microsatellite polymorphisms in genomic
DNA prepared from Ewing sarcoma tumor specimens as compared to non-afflicted European
and African normal genomic DNA.
- To determine if GGAA-microsatellite polymorphisms at specific loci in genomic DNA
prepared from Ewing's sarcoma tumor specimens correlates with disease outcome in
patients treated on COG protocol AEWS0031.
- To determine whether whole-genome amplification introduces alterations in
GGAA-microsatellites as compared to non-amplified genomic DNA.
OUTLINE: Genomic PCR is used to amplify the microsatellites in the NR0B1 and GSTM4
promoters. In addition to determining microsatellite size, each microsatellite is sequenced
following cloning into the pCR4 vector (Invitrogen) using standard topoisomerase cloning
Stephen Lessnick, MD, PhD
University of Utah
United States: Federal Government