Genomic Signatures of Malignant Germ Cell Tumor Progression: A Retrospective Study of Banked Specimens
- Explore inter-tumoral heterogeneity in DNA methylation by tumor histology.
- Determine the genomic methylation pattern in the tumors.
- Correlate methylation pattern with tumor histology and clinical characteristics.
- Carry out exome capture and massively parallel sequencing on selected germ cell tumors
(GCTs) and matched normal tissue.
- Perform exome capture and Solexa sequencing on a selected set of GCTs.
- Validate candidate mutations in an independent set of tumors.
OUTLINE: Archived blood and tumor tissue samples are analyzed for genomic methylation
pattern, exome capture and sequencing, and candidate mutations by methylation-specific PCR
techniques, single nucleotide polymorphism (SNP) arrays, and Solexa sequencing methods.
Results are validated by using pyrosequencing assays and primer-extension assays.
Methylation pattern is also associated with each patient's tumor histology and clinical
James F. Amatruda, MD, PhD
Simmons Cancer Center