DNA Methylation-based Assays for Detecting Disease Spread in Rhabdomyosarcoma
- To establish a panel of hypermethylation assays for minimal disseminated disease
analysis in rhabdomyosarcoma (RMS).
- To apply the hypermethylation assays to a series of bone marrow specimens from RMS
OUTLINE: Archived tumor, bone marrow, and peripheral blood samples are analyzed for DNA
hypermethylation by PCR-based assays and microarray assays. Results are then compared with
each patient's clinical and pathological characteristics, and outcomes. Patterns of locus
hypermethylation in tumor and paired bone marrow samples are also compared.
Panel of hypermethylation assays for minimal disseminated RMS
Stephen R. Master, MD
University of Pennsylvania
United States: Federal Government