Analysis of Intersectin Expression in Primary Human Neuroblastomas
- To determine whether intersectin (ITSN1) proteins are present at elevated levels in
primary human tumor samples.
OUTLINE: Archived tumor tissue samples are analyzed for intersectin (ITSN1) expression by
western blot assays with enhanced chemilumiscence and exposed to X-ray film. The films are
scanned, and levels of ITSN1 signal are quantified by densitometry.
ITSN1 is highly expressed in neuroblastoma tumors and plays an important role in the development/progression of these tumors
John P. O'Bryan, PhD
University of Illinois
United States: Federal Government