Trial Information

The Molecular Mechanisms of Transformation in Peripheral T-cell and NK Lymphomas

OBJECTIVES:

- To identify mutations in oncogenes and tumor suppressors of peripheral T-cell lymphoma
or natural killer (NK) cell neoplasm.

- To delineate the mechanisms that mediate oncogenic effects and their clinical
prognostic significance in these diseases.

- To perform a comprehensive mutation analysis via whole exome resequencing in a panel of
peripheral T-cell or NK lymphomas.

OUTLINE: Fixed paraffin-embedded tumor tissue samples are analyzed for mutations by the
Illumina HiSeq platform. Sequencing reads are performed by GAPipeline version 1.5.1 and
mapping on the human genome performed using the MAQ software version 0.7.1. Genes harboring
validated somatic mutations are then amplified by PCR and analyzed by Sanger resequencing.