Inclusion Criteria:

1. men and women

2. age over 18 y.o.

3. signed informed consent form.

4. Patients barriers of Non-small Cell Lung Cancer Metastatic

5. chemotherapy non yet begun

6. patients barrier of CTC

7. Research for mutation of EGFR and Kras made on the primitive tumor

8. Man and woman old enough to procreate under effective contraceptives

Exclusion Criteria:

1. small cells bronchial cancer

2. Non metastatic bronchial cancer

3. Absence of detectable CTC at the patient

4. Private individuals of freedom or under tutelage (including legal guardianship)

5. Incapacity to sign the inform consent form or psychiatric, behavioural disorders or
geographical situation precluding the follow-up of the protocol

6. Pregnancy, lactating or refusal of the contraception for women