Inclusion Criteria:

1. age: 22-48 years old

2. gender: male, female

3. who got a depressive score above 20, were included in the depressed group

4. those who obtained a depressive score below 9, were chosen and they were included in
the healthy control group.

5. the healthy control group to match to the depressed patients for education, social
occasion, occupational and economical situation.

6. the depression disorder was confirmed on the basis of the DSM criteria

Exclusion Criteria:

1. present or past psychiatric

2. physical diagnoses

3. drug consumption

4. use of narcotic substances

5. physical and psychosomatic disorders

6. the stressor

7. malfunction of thyroid gland

8. shift working